Nano-Honeycomb Electrode-Based QCM Sensor and Its Application for PPI Detection
| Autor: | Takaaki Sumiyoshi, Nozomi Kazama, Takeshi Ito, Naoto Asai, Tomohiro Shimizu, Yasuo Nagaoka, Shoso Shingubara, Naohiro Matsumoto |
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| Rok vydání: | 2019 |
| Předmět: |
Analyte
Materials science business.industry 010401 analytical chemistry technology industry and agriculture Aluminon Quartz crystal microbalance 01 natural sciences 0104 chemical sciences Anode Crystal chemistry.chemical_compound chemistry Electrode Nano Optoelectronics Electrical and Electronic Engineering business Instrumentation Quartz |
| Zdroj: | IEEE Sensors Journal. 19:4025-4030 |
| ISSN: | 2379-9153 1530-437X |
| DOI: | 10.1109/jsen.2019.2898408 |
| Popis: | An anodic aluminon oxide-based nano-honeycomb electrode was fabricated on a quartz crystal to increase the surface area binding with an analyte for using as a sensing device in a quartz crystal microbalance to monitor the protein–protein interactions (PPIs). As examples, we detected PPIs of anti-Bcl-2 and Bcl-2, and Bcl-2 and Bax in real time. A sensor with a flat Au electrode showed a tiny frequency shift upon sample injection. However, our fabricated device could detect Bcl-2 and Bax in sequence. Frequency shifts corresponding to the binding of Bcl-2 to anti Bcl-2 and that of Bax to Bcl-2 increased linearly. |
| Databáze: | OpenAIRE |
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