| Popis: |
Amperometric bienzyme sensors were constructed based on solid graphite electrodes modified with polymer redox mediator, horseradish peroxidase and a variety of oxidases and covered with a bipolar electrostatic protective layer. Glucose, ethanol, choline, glycero-3-phosphate and cholesterol sensors were designed and operated at 0 mV vs. AgAgCl using single potential step chronoamperometry. The protection of the sensor against interference from ascorbic acid and linearity of response were provided by a bipolar electrostatic layer. Sensitivities as high as 0.74 μA cm−2 mM−1 of glucose, 0.71 μA cm−2 mM−1 of ethanol 5.7 μA cm2 mM−1 of glycerol-1-phosphate, and 28.3 nA cm−2 per 10 μM of choline, were achieved. |
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