EARLY RESPONSE OF INTESTINAL PROGLUCACON AND ODC mRNAs DURING ADAPTATION TO JEJUNECTOMY
| Autor: | P. K. Lund, S. E. Selub, R Fuller, Dorothy B. Rountree, Martin H. Ulshen |
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| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | Pediatric Research. 27:543-543 |
| ISSN: | 1530-0447 0031-3998 |
| Popis: | Locally produced peptide hormones may mediate adaptive growth of ileum after jejunectomy. Previously, we found that proglucagon (P) mRNA is elevated in adapting ileum at 1 and 5 wk after jejunectomy. A transient rise in mucosal ornithine decarboxylase (ODC) activity identifies early adaptation. In this study, we contrasted patterns of abundance of P and ODC mRNAs in remnant; ileum early after 80% resection of proximal small bowel. Controls had transection and anastomosis. Animals were fasted for 24 h after surgery and then pair-fed. Ileum was removed at 4, 12, 24, 28, 36, 48 and 96 h after surgery; poly A+ RNAs were analysed by quantitative Northern blot hybridizations using rat P cDNA and synthetic ODC oligomer probes. Abundance of P and ODC mRNAs in Ileum of resected animals tripled that of controls by 12 h after surgery; P mRNA remained elevated up to 96 h after surgery while ODC mRNA fell to control levels by 36-48 h. Conclusion: After jejunectomy, there are rapid increases in P and ODC mRNAs, independent of enteral nutrient. Whereas the rise in ODC mRNA is transient and brief, the elevation in P mRNA persists for weeks. This pattern of abundance of P mRNA is consistent with a role of one or more P mRNA products as a mediator of adaptive growth of bowel. |
| Databáze: | OpenAIRE |
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