Cryopreservation of Black Bengal buck semen: Effects of diluents and freezing on sperm motility and morphology
| Autor: | Sadia Afroz, Quzi Sharmin Akter, M.A.M. Yahia Khandoker, Md. Rashedul Islam |
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| Rok vydání: | 2008 |
| Předmět: |
Tris
endocrine system Cryoprotectant urogenital system Artificial insemination medicine.medical_treatment Semen General Medicine Biology Diluent Sperm Cryopreservation Andrology chemistry.chemical_compound fluids and secretions chemistry medicine General Agricultural and Biological Sciences Sperm motility |
| Zdroj: | Animal Science Journal. 79:550-553 |
| ISSN: | 1740-0929 1344-3941 |
| Popis: | Semen from Black Bengal bucks was collected to establish a cooling protocol (to −196°C) for buck semen preservation, and to study the effect of freezing on sperm motility and morphology. Semen was diluted with diluents (Triladyl & Tris) and cryoprotectants, filled into straws, sealed, cooled (to 5°C) and equilibrated. After dilution, motility ranged from 75.00% to 76.67% and from 73.33% to 80.00% in Triladyl and Tris diluents, respectively. Motility of sperm after cooling to 5°C in Triladyl and Tris diluents ranged from 65.00% to 66.67% and from 63.33% to 70.00%, respectively. After equilibration in straws, the semen was subjected to a freezing protocol in a computer-controlled biofreezer CL-3000 (cooling at 10°C per minute, from 5°C to −80°C) and plunged into liquid nitrogen. Sperm motility of re-thawed semen varied from 38.33% to 43.33% and from 6.00% to−6.67% in Triladyl and Tris diluents, respectively. Sperm morphology of re-thawed semen was studied and head damage or cryoinjury was found in 2–3% of sperm in Triladyl diluents and 3–6% in Tris diluents. Whether the differences of sperm motility and head damage reflect fertility after artificial insemination is yet unknown and needs to be studied further. |
| Databáze: | OpenAIRE |
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