Culture-Independent Estimation of Optimal and Maximum Growth Temperatures of Archaea in Subsurface Habitats Based on the G+C Content in 16S rRNA Gene Sequences

Autor: Jun-ichiro Ishibashi, Toshiro Yamanaka, Hiroyuki Kimura, Kousuke Mori, Tomokazu Tashiro, Satoshi Hanada, Kenji Kato
Rok vydání: 2010
Předmět:
Zdroj: Geomicrobiology Journal. 27:114-122
ISSN: 1521-0529
0149-0451
DOI: 10.1080/01490450903456699
Popis: The temperature ranges of growth of archaea are strongly correlated with the guanine-plus-cytosine (G+C) contents of their 16S rRNA sequences (P GC ). In order to estimate minimum (T min ), optimal (T opt ), and maximum (T max ) growth temperatures of uncultured archaea based on P GC , the 16S rRNA gene sequences of 207 archaeal species were collected from public databases, and their T min , T opt and T max were extracted from description papers and reviews. These values of growth temperatures were plotted against P GC , and then the regression lines for estimating T min , T opt and T max were calculated. We PCR-amplified the archaeal 16S rRNA gene fragments from the hot water samples, cloned the fragments, and determined the sequences. Growth temperatures of environmental archaea were inferred from G+C content of the 16S rRNA gene sequences by the regression lines. In the terrestrial hot springs (74°C and 85°C), both estimated growth temperatures of archaea were higher than in situ temperatures of hot sp...
Databáze: OpenAIRE
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