Quantitation of red cell-bound IgG by an enzyme-linked antiglobulin test in the patients with warm-type autoimmune haemolytic anaemia

Autor:	H Łopieńska, J Fabijańska-Mitek, A Adamowicz-Salach, R Pogłod
Rok vydání:	2006
Předmět:	chemistry.chemical_classification Red Cell business.industry Anemia Autoantibody Hematology Haemolysis medicine.disease Red blood cell medicine.anatomical_structure Enzyme chemistry Immunology Medicine IgG Autoantibody C3 complement business
Zdroj:	Clinical & Laboratory Haematology. 28:241-244
ISSN:	0141-9854
DOI:	10.1111/j.1365-2257.2006.00798.x
Popis:	We have previously reported the evaluation of a gel-direct antiglobulin test and enzyme-linked antiglobulin test (ELAT) in the laboratory diagnosis of autoimmune haemolytic anaemia (AIHA). We now report our experience with quantitative ELAT performed on a large group of patients under long-term observation. The number of IgG molecules/red blood cell was determined in 658 blood samples from 268 randomly selected patients with warm-type AIHA. Eighty-six patients were tested every 2-4 weeks for several months. Laboratory signs of haemolysis were present in 65.7% of blood samples with a small amount of red cell-bound autoantibody ( 1000 IgG molecules/red blood cell, which were predominantly IgG3 and C3 complement, the qualitative factors that may increase haemolysis. In 79% of periodically tested patients, the number of IgG autoantibody molecules/red blood cell decreased and this correlated with the improvement of haemolysis parameters. The number of IgG molecules varied in 21% of AIHAs and was associated with poor prognosis.
Databáze:	OpenAIRE
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