| Popis: |
The regenerative nodule in experimental rat cirrhosis induced by a choline deficient diet is defined as a cell mass projected on the surface of the fibrous liver. On microscopic examination, the cell masses are consisted of a small non-fatty or less fatty cells alined in one cell thick plate. They are the cells of homogenous in the property with reduced succinic dehydrogenase activity. Occasionally another type of the regeneration nodules was also encountered. The nodules are consisted of the cells of not homogeneous in the property. A large cells having a large nuclei with more than two nucleoli are arranged in rather odd fashion. Thus having a structure of adenoma. Both types of the regenerative nodules formed in fatty liver cirrhosis and proliferate expansively. Customarilly, such newly formed cell mass have been designated as the “regenerative” nodele. However, the mechanism operating the proliferation of the cells has recently debated by some investigators. A comparative study has been attempted in connection with the study of hepatic cell proliferation after partial hepatectomy in the normal liver by using a cytochemical method, tracing method using radioisotope P32, autoradiography using tritiated thymidine, however, no report came out to date for the elucidation of the subject using microspectrophotometry. The results obtained here were based on a study applied a microspectrophotometric method for the determination of DNA content of both the cells of regenerative nodules and internodular areas. |
