Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an antiestrogen in MCF-7 human breast cancer cells
| Autor: | T.R. Narisimhan, V. Krishnan, S.H. Safe |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Environmental Engineering Chemistry Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Cathepsin D Radioimmunoassay General Medicine General Chemistry Antiestrogen Pollution In vitro Cytosol Endocrinology MCF-7 Internal medicine Cancer cell medicine Environmental Chemistry Intracellular |
| Zdroj: | Chemosphere. 25:947-950 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/0045-6535(92)90089-a |
| Popis: | The effects of 1 nM 17β-estradiol, 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and their combination on the formation of intracellular cytosolic 52- and 34-kDa proteins was determined using a commercially available cathepsin D radioimmunoassay procedure (ELSA cath-D™ kit) which recognizes both the 34- and 52-kDa proteins. In control (ethanol-treated) cells, the 52-kDa protein levels were 305 ± 49 fmol/mg protein (100%) whereas in cells treated with 1 nM 17β-estradiol, 1 nM TCDD or their combination the levels were 1223 ± 142 (401%), 478 ± 54 (150%) and 227 ± 33 (74%). These results confirm that TCDD inhibits the 17β-estradiol-induced intracellular formation of the 52-/34-kDa proteins and further extends the antiestrogenic properties of this compound. |
| Databáze: | OpenAIRE |
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