Accumulation of domoic acid by the sea scallop (Placopecten magellanicus) fed cultured cells of toxic Pseudo-nitzschia multiseries
| Autor: | E R Kenchington, W Silvert, R Pocklington, B Bradford, C J Bird, D J Douglas |
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| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Canadian Journal of Fisheries and Aquatic Sciences. 54:907-913 |
| ISSN: | 1205-7533 0706-652X |
| DOI: | 10.1139/f96-333 |
| Popis: | Sea scallops (Placopecten magellanicus) were fed Pseudo-nitzschia multiseries (formerly P. pungens f. multiseries, Nitzschia pungens f. multiseries) cells of high domoic acid (DA) content (4.0-6.7 pg DA cdot cell-1) for 22 days, followed by 14 days of feeding with nontoxic microalgae. DA was incorporated within 24 h by the scallops, with increased uptake after 6 days, and was concentrated in tissues in the following order: digestive gland >> remaining soft tissue >> adductor muscle. A maximum DA concentration of 3108 mu g cdot g-1 was recorded in the digestive gland, approximately 150 times the regulatory limit (20 mu g DA cdot g-1) and among the highest levels observed in bivalve molluscs; however, only trace amounts, 0.7-1.5 mu g cdot g-1, were found concomitantly in the adductor muscle. At the end of the exposure period, 50.9% of the DA that had been supplied to the scallops had been incorporated into the tissues. Concentrations in the digestive gland 14 days after termination of the toxic diet remained high, 752 mu g DA cdot g-1. Throughout the experiment, there was no sign of illness or mortality attributable to high DA loading, although the destructive sampling of animals did not allow us to assess the effects of the toxin in the longer term. |
| Databáze: | OpenAIRE |
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