Autor: Simona Fiorentini, Adolfo Turano, Maria Rosa Bani, Fabio Landoni, Arnoldo Caruso, Stefano Maria Giulini, Luciano Zardi, Gerardo Zanetta, Romina Dossi, Stefano Bonardelli, Renato G. S. Chirivi, Raffaella Giavazzi, Pierpaolo Graziotti, Giulio Alessandri
Rok vydání: 1999
Předmět:
Zdroj: Clinical and Experimental Metastasis. 17:655-662
ISSN: 0262-0898
Popis: We recently developed a method for the isolation and purification of tumour-derived endothelium. In this study the phenotypic and functional properties of human tumour-derived microvascular endothelial cells (TdMEC) were examined. Endothelium obtained from human adrenal gland specimens (HAMEC) was used as a reference microvascular endothelial cell population. TdMEC formed a confluent monolayer with the typical morphological appearance of endothelium and were positive for endothelial markers such as Ulex-1 lectin, CD31 antigen, von Willebrand Factor and VE-cadherin. The addition of acidic Fibroblast Growth Factor (aFGF), basic FGF (bFGF) or Vascular Endothelial Growth Factor (VEGF) substantially improved proliferation of TdMEC; and kidney carcinoma derived endothelial cells were more responsive to FGFs, whereas glioblastoma derived endothelial cells greatly responded to VEGF. TdMEC expressed high levels of the VEGF receptors, KDR/flk-1 and Flt-1, as shown by northern blot analysis. TdMEC expressed the adhesion molecules ICAM-1, VCAM-1 and E-selectin that could be further increased by exposing TdMEC culture to interleukin-1. All the TdMEC expressed interleukin-8 mRNA. These findings show that TdMEC in vitro maintain several of the features described for microvasculature. Thus, TdMEC represent a useful tool to study markers for tumor vasculature.
Databáze: OpenAIRE
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