| Popis: |
Multiphoton microscopy has become an ubiquitous research tool for neuroscience, disease studies, embryology and immunology. Refinements in non-linear imaging techniques have gone hand-in-hand with improvements in tuning range, power, pulse management and other features of femtosecond lasers specifically designed for these applications. While potential clinical applications require lasers with multiple outputs able to support multimodal imaging, recent developments in neuronal imaging like 3-photon microscopy or optogenetic activation require higher energies per pulse and longer wavelengths than conventional multiphoton microscopy. Here we describe the design and characteristics of laser sources able to address all these applications, staring with the more established ultrafast laser technology based on Titanium Sapphire lasers and their OPOs and covering recently developed sources like Ytterbium fiber mode-locked lasers and amplifiers with their tunable wavelength extensions. |
