Thermomechanical responses of microfluidic cantilever capture DNA melting and properties of DNA premelting states using picoliters of DNA solution
| Autor: | Parth Rakesh Desai, Thomas Thundat, Javix Thomas, Keren Jiang, Siddhartha Das, Arindam Phani, Faheem Khan |
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| Rok vydání: | 2019 |
| Předmět: |
010302 applied physics
Cantilever Materials science Physics and Astronomy (miscellaneous) Microfluidics 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Heat capacity Premelting Nucleic acid thermodynamics Chemical physics 0103 physical sciences Thermophysics Melting point A-DNA 0210 nano-technology |
| Zdroj: | Applied Physics Letters. 114:173703 |
| ISSN: | 1077-3118 0003-6951 |
| DOI: | 10.1063/1.5092333 |
| Popis: | Melting or thermal denaturation of a DNA molecule and the different bubble-rich, premelting DNA states that serve as a precursor for DNA thermal denaturation are vital events in DNA thermophysics. In this study, we employ cantilever-based sensing to firstly pinpoint the occurrence of DNA melting and identify the temperature Tm characterizing the melting. Very importantly, this sensing is carried out with an extremely small volume (∼picoliters) of DNA sample with the cantilever demonstrating an extremely high sensitivity on the order of mJ / g ⋅ K corresponding to pico-Joules of energy input. Secondly, this same large sensitivity of the cantilever is used to quantify the hitherto unknown thermophysical properties of the bubble-rich DNA premelting states. In fact, for both the melting and premelting states, the cantilever provides a framework to calculate the specific heat capacity and the storage and loss moduli of the cantilever-DNA-solution system, thereby establishing a platform for quantifying DNAs' thermo-mechanical behavior. |
| Databáze: | OpenAIRE |
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