| Autor: |
Elisavet Ioannou, Christina Varotsou, Nikolaos E. Labrou, Nikolaos Georgakis, Georgios Premetis, Evangelia G. Chronopoulou |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781071601624 |
| DOI: |
10.1007/978-1-0716-0163-1_15 |
| Popis: |
Ligand fishing is a convenient bioanalytical screening method that is based on the affinity selection of a ligand from a complex biological sample by an immobilized receptor. It is a versatile affinity-based screening approach and it has found application in multiple interacting pairs such as enzyme-inhibitor/activator, antigen-antibody, receptor-ligand, and protein-protein. Important parameters that affect the successful operation of the method are the high specificity and strong binding affinity of the interacting pair (e.g., enzyme-ligand complex) and the elution of the bound ligand from the complex. This chapter provides protocols for the synthesis of affinity adsorbent and its application in off-line ligand-fishing procedure for a 6His-tagged glutathione transferase (GST). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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