o-Phthalyl amidase in the synthesis of loracarbef: Process development using this novel biocatalyst

Autor:	Surya Vangala, Barbara Shreve Briggs, Thomas D. Black, Robert S. Evans, Muth William Lawrence, Milton J. Zmijewski
Rok vydání:	1996
Předmět:	Reaction mechanism Xanthobacter agilis Stereochemistry Chemistry Bioengineering General Medicine Applied Microbiology and Biotechnology Combinatorial chemistry Amidase Hydrolysis Biocatalysis medicine Phthalyl amidase Loracarbef Biotechnology medicine.drug Antibacterial agent
Zdroj:	Biotechnology Letters. 18:875-880
ISSN:	1573-6776 0141-5492
DOI:	10.1007/bf00154613
Popis:	A dephthalylation step utilizing a novel enzyme, o-phthalyl amidase, was developed. This step was part of a potentially new large scale synthetic route for a novel beta-lactam antibiotic Loracarbef. The enzyme was isolated from the organism Xanthobacter agilis. Purification of the enzyme to near homogeneity was accomplished by a 3-step procedure. Studies indicated that the phthalimido group can be opened chemically to generate the o-phthalyl derivative. This enzyme then can remove the phthalyl group from o-phthalylated amides. Optimization of the process was achieved by combining these two hydrolysis steps. Conversion yields of 85–97.8% (mol/mol) were obtained from reactions at substrate concentrations of 5–10% (w/v).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::04cfe227387c7c0634fbcdb9d216e77d https://doi.org/10.1007/bf00154613 Zobrazit plný text záznamu Full text from SpringerLink