16SrIII-J phytoplasmas infecting lettuce and Swiss chard crops in Chile
| Autor: | Javiera Molina, Camila Gamboa, Alan Zamorano, Nicola Fiore, Marcelo Bustamante, Nicolás Quiroga |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) Cloning biology fungi food and beverages Swiss Chard Cell Biology biology.organism_classification 01 natural sciences food.food 03 medical and health sciences Horticulture 030104 developmental biology Infectious Diseases food Phytoplasma Plant species Parasitology Restriction fragment length polymorphism Nested polymerase chain reaction Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Phytopathogenic Mollicutes. 7:91 |
| ISSN: | 2249-4677 2249-4669 |
| Popis: | During spring 2016 commercial lettuce and Swiss chard plants, exhibiting disease symptoms suggestive of possible phytoplasma infection, were collected together with asymptomatic plants in Metropolitana and Arica y Parinacota regions of Chile, respectively. Nucleic acids extracted from leaf midribs were used for phytoplasmas detection. Nested polymerase chain reaction allowed the amplification of bands of the expected size only from symptomatic samples. Restriction fragment length polymorphism analysis, cloning, and sequencing allowed the identification of 16SrIII-J phytoplasmas in both plant species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|