| Autor: |
Nobuo Kobayashi, Yosuke Ueda, Hiroyuki Itamura, Chihiro Ishibashi, Akira Nakatsuka, Masumi Yamagishi, Tsuyoshi Maruo |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Postharvest Biology and Technology. 62:89-92 |
| ISSN: |
0925-5214 |
| Popis: |
Persimmon ( Diospyros kaki Thunb.) fruit undergoes intensive cell wall modification during postharvest fruit softening. Xyloglucan metabolism is important in cell wall disassembly. We cloned cDNAs for two xyloglucan endotransglycosylase/hydrolase genes ( DkXTH 1 and DkXTH 2) from ‘Saijo’ persimmon fruit treated with dry ice to remove astringency. In order to determine the ethylene dependence of XTH gene expression, fruit were exposed to 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action, prior to removal of astringency. Ethylene production increased in mature control and 1-MCP-pretreated fruit after dry-ice treatment, and flesh firmness decreased to the same extent during dry-ice treatment in the control and 1-MCP-pretreated fruit. After dry-ice treatment, control fruit softened completely, but fruit firmness was maintained in 1-MCP-pretreated fruit. Accumulation of DkXTH 1 mRNA was induced simultaneously with commencement of ethylene production in mature control fruit. Pretreatment with 1-MCP delayed accumulation of DkXTH 1 mRNA. DkXTH 2 expression also coincided with fruit softening but was intensified by 1-MCP treatment during the deastringency treatment. These results indicate that fruit softening was related to both DkXTH 1 and DkXTH 2 expression in ‘Saijo’ persimmons. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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