Multiple organ gigantism caused by mutation in VmPPD gene in blackgram (Vigna mungo)
| Autor: | Akito Kaga, Kumi Hirano, Yu Takahashi, Charaspon Thavarasook, Norihiko Tomooka, Kyoko Takagi, Ken Naito, Bubpa Chaitieng, Eri Ogiso-Tanaka, Masao Ishimoto |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Cloning Mutation Mutant Wild type food and beverages Plant Science Meristem Biology medicine.disease_cause biology.organism_classification 01 natural sciences Phenotype Vigna 03 medical and health sciences 030104 developmental biology Botany medicine Agronomy and Crop Science Gene 010606 plant biology & botany |
| Zdroj: | Breeding Science. 67:151-158 |
| ISSN: | 1347-3735 1344-7610 |
| Popis: | Seed size is one of the most important traits in leguminous crops. We obtained a recessive mutant of blackgram that had greatly enlarged leaves, stems and seeds. The mutant produced 100% bigger leaves, 50% more biomass and 70% larger seeds though it produced 40% less number of seeds. We designated the mutant as multiple-organ-gigantism (mog) and found the mog phenotype was due to increase in cell numbers but not in cell size. We also found the mog mutant showed a rippled leaf (rl) phenotype, which was probably caused by a pleiotropic effect of the mutation. We performed a map-based cloning and successfully identified an 8 bp deletion in the coding sequence of VmPPD gene, an orthologue of Arabidopsis PEAPOD (PPD) that regulates arrest of cell divisions in meristematic cells. We found no other mutations in the neighboring genes between the mutant and the wild type. We also knocked down GmPPD genes and reproduced both the mog and rl phenotypes in soybean. Controlling PPD genes to produce the mog phenotype is highly valuable for breeding since larger seed size could directly increase the commercial values of grain legumes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|