mRNA D2 Dopaminergic Receptor Expression after Hypoxia-Ischemia in Rat Immature Brain

Autor:	Annie-Laure Suc, Sylvain Cantagrel, J. Laugier, Denis Guilloteau, Sylvie Bodard, Pierre Gressens, Sylvie Chalon
Rok vydání:	2001
Předmět:	medicine.medical_specialty Receptor expression Dopaminergic Ischemia Striatum Hypoxia (medical) Biology medicine.disease Endocrinology Dopamine receptor Dopamine receptor D2 Internal medicine Pediatrics Perinatology and Child Health medicine medicine.symptom Receptor Developmental Biology
Zdroj:	Neonatology. 80:68-73
ISSN:	1661-7819 1661-7800
DOI:	10.1159/000047123
Popis:	Previous studies have shown a reduction of dopaminergic D2 receptors (D2R) in the striatum after hypoxia-ischemia in newborn rats. We show here an early and transient reduction of mRNA D2R in nonatrophic brains following hypoxia-ischemia. The left carotid artery of P7 rats was ligated followed by hypoxia for 2 h. The rats were sacrificed after 24 h, 48 h and 14 days. D2R mRNA was studied by in situ hybridization, the cell number by conventional histology, and neuronal and astrocyte differentiation by immunohistochemistry. A 20% reduction of striatal mRNA D2R occurred 24 h after hypoxia-ischemia, whereas no reduction was observed after 48 h and 14 days. There were no differences in total cell number and in the expression of neuronal (MAP-1, MAP-2) and astrocyte (GFAP) markers between both brain hemispheres nor between control and hypoxia-ischemia animals. The early decrease in mRNA D2R could explain the delayed reduced D2R after neonatal hypoxia-ischemia.
Databáze:	OpenAIRE
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