| Popis: |
Affinity chromatography has been demonstrated to be a powerful tool for the isolation and purification of parasite proteins and has potential applications for diagnosis and therapy. Many studies have focused on parasite proteins that modulate host cell defense, as gp63, a glycoprotein from Leishmania spp., that is involved in the cleavage of the complement factor C3b to iC3b, which promotes adhesion of promastigotes to macrophages via complement receptor 3 (Brittgham et al., 1995). This route of internalization does not lead to production of oxygen radicals or NO and favors parasite subsistence within the host cell. Another example is the cysteine protease B (CPB), an important virulence factor of the Leishmania (L.) mexicana complex, that inhibits lymphocytes Th1 and/or promotes the Th2 response either through proteolytic activity or through epitopes derived from its COOH-terminal extension (Pereira et al., 2011). |
