Localization of Tyrosine Phosphorylated Proteins in Human Sperm and Relation to Capacitation and Zona Pellucida Binding1
| Autor: | Françoise Urner, H. Lucas, Denny Sakkas, Greet Leppens-Luisier, D. Chardonnens, A. Campana, Daniel R. Franken |
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| Rok vydání: | 2003 |
| Předmět: |
endocrine system
urogenital system Tyrosine phosphorylation Cell Biology General Medicine Biology Sperm Cell biology chemistry.chemical_compound medicine.anatomical_structure Human fertilization Reproductive Medicine chemistry Capacitation Immunology medicine Phosphorylation Protein phosphorylation Tyrosine Zona pellucida reproductive and urinary physiology |
| Zdroj: | Biology of Reproduction. 68:1463-1469 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.102.011023 |
| Popis: | Mammalian sperm must undergo a process known as capacitation before fertilization can take place. A key intracellular event that occurs during capacitation is protein tyrosine phosphorylation. The objective of this study was to investigate and visualize protein tyrosine phosphorylation patterns in human sperm during capacitation and interaction with the zona pellucida. The presence of specific patterns was also assessed in relation to the fertilizing capacity of the spermatozoa after in vitro fertilization. Protein tyrosine phosphorylation was investigated by immunofluorescence. Phosphorylation increased significantly with capacitation and was localized mainly to the principal piece of human sperm. Following binding to the zona pellucida, the percentage of sperm with phosphotyrosine residues localized to both the neck and the principal piece was significantly higher in bound sperm than in capacitated sperm in suspension. When the percentage of principal piece-positive sperm present after capacitation was |
| Databáze: | OpenAIRE |
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