MS1-type bioengineered spider silk nanoparticles do not exhibit toxicity in anin vivomouse model

Autor:	Andrzej Mackiewicz, Andrzej Marszałek, Karolina Penderecka, Hanna Dams-Kozlowska, Anna Florczak, Tomasz Deptuch, Ewa Leporowska, Anna Lewandowska
Rok vydání:	2021
Předmět:	Biodistribution Biocompatibility medicine.diagnostic_test Chemistry fungi Biomedical Engineering Medicine (miscellaneous) Complete blood count Bioengineering macromolecular substances Development Pharmacology SILK In vivo Toxicity medicine Animal mortality General Materials Science Spider silk
Zdroj:	Nanomedicine. 16:1553-1565
ISSN:	1748-6963 1743-5889
DOI:	10.2217/nnm-2021-0029
Popis:	Background: Due to factors such as silk sequence, purification, degradation, morphology and functionalization, each silk variant should be individually tested for potential toxicity. Aim: In vivo toxicological evaluation of the previously characterized bioengineered H2.1MS1 spider silk particles that can deliver chemotherapeutics to human epidermal growth factor receptor 2-positive breast cancer. Materials & methods: Silk nanoparticles (H2.1MS1 and control MS1) were administered intravenously to mice, and then the organismal response was assessed. Several parameters of acute and subchronic toxicity were analyzed, including animal mortality and behavior, nanosphere biodistribution, and histopathological analysis of internal organs. Also, the complete blood count, as well as the concentration of biochemical parameters and cytokines in the serum, were examined. Results & conclusion: No toxicity of the systemically administrated silk nanosphere was observed, indicating their potential application in biomedicine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::048947d9e686d324e2a108a9f13ad46f https://doi.org/10.2217/nnm-2021-0029 Zobrazit plný text záznamu