Analysis of intracutaneous inflammatory lesions with skin blisters
| Autor: | Pekka Häyry, U. Kiistala, Maija Horsmanheimo, von Willebrand E, Tötterman Th |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
integumentary system
business.industry Tetanus medicine.medical_treatment Immunology Toxoid Tuberculin chemical and pharmacologic phenomena Blisters medicine.disease complex mixtures In vitro Pathology and Forensic Medicine Antigen medicine Immunology and Allergy medicine.symptom skin and connective tissue diseases Receptor business Saline |
| Zdroj: | Clinical Immunology and Immunopathology. 12:443-452 |
| ISSN: | 0090-1229 |
| DOI: | 10.1016/0090-1229(79)90049-7 |
| Popis: | Blisters 0.1 cm3 in volume were generated by vacuum onto the abdominal skin. Twelve blisters received intradermally an 1 12 skin-reactive dose of purified protein derivative of tuberculin (PPD) underneath the blister bottom; 12 control blisters located at the contralateral side received an equivalent volume of saline. The cells recovered from the PPD blisters on the third day after the antigen injection responded in a prompt and strong fashion to PPD but gave ordinary slow primary responses to oidiomycin and tetanus toxoid. Blood leukocytes or cells recovered from the control blisters responded to all test antigens in the slow primary fashion. Approximately 50% lymphocytes present in the PPD blisters of strongly Mantouxpositive individuals bound 3H-labeled PPD compared to 0.01–0.05% lymphocytes binding 3H-labeled oidiomycin or tetanus toxoid under the same conditions. The relative number of PPD-binding lymphocytes in PPD blisters, one-third of these being T cells, was 100- to 1000-fold compared to control blisters or to the blood. These results demonstrate a specific accumulation of antigen-reactive lymphocytes with receptors for the inducing antigen in situ, at the site of immune inflammation. The blister method provides a feasible basis for the recovery of these cells in a functionally viable state. |
| Databáze: | OpenAIRE |
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