A new sub-sampling method for analysis of air samples collected with the Andersen single-stage sampler

Autor: Catharine M. Catranis, Shuang Zhou, Analie Fernando, Shannon R. Morey, Susan E. Anagnost, C. J. K. Wang, Lianjun Zhang
Rok vydání: 2006
Předmět:
Zdroj: Aerobiologia. 22:177-184
ISSN: 1573-3025
0393-5965
DOI: 10.1007/s10453-006-9030-2
Popis: A protocol for bioaerosol collection was developed that provides not only accurate predictions of fungal concentration, but also improves species recovery. Random transfer of a subset of 50 of the 400 impaction points from Andersen single-stage bioaerosol sampling plates results in subcultures that are accurate predictors of fungal concentration (CFU/m3), when compared to duplicate untouched Andersen plates. A linear regression model was developed to estimate CFU/m3 from the colonies counted on the Random-50 plates. The random transfer to five plates (“Random-50” plates), allows large numbers of fungi to be recovered and identified, including slow-growing fungi that otherwise would be masked by fast-growing fungi.
Databáze: OpenAIRE