Preparation and characterization of magnetic Fe3O4/CRGO nanocomposites for enzyme immobilization
| Autor: | Hai-xia Wu, Xiao-chen Wu, Yan Zhang, Cong-yu Wu |
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| Rok vydání: | 2012 |
| Předmět: |
Nanocomposite
Materials science Immobilized enzyme biology Scanning electron microscope Graphene Metals and Alloys Oxide Geotechnical Engineering and Engineering Geology Condensed Matter Physics Horseradish peroxidase law.invention Catalysis chemistry.chemical_compound chemistry law Polymer chemistry Materials Chemistry biology.protein Powder diffraction Nuclear chemistry |
| Zdroj: | Transactions of Nonferrous Metals Society of China. 22:s162-s168 |
| ISSN: | 1003-6326 |
| DOI: | 10.1016/s1003-6326(12)61703-8 |
| Popis: | A one-step hydrothermal procedure to form Fe 3 O 4 nanospheres on chemically reduced graphene oxide (CRGO) surfaces was proposed, and these nanocomposites were used as substrates for enzyme immobilization. The as-prepared Fe 3 O 4 /CRGO nanocomposites were characterized using scanning electron microscopy (SEM), X-ray powder diffraction (XRD), FT-IR and vibrating sample magnetometer. Fe 3 O 4 microspheres are randomly distributed on graphene sheets, and the average diameter of Fe 3 O 4 microspheres is about 260 nm. Horseradish peroxidase (HRP) was used as a model enzyme to investigate the immobilization activity. The HRP loading was 23.3 mg/g supports and retained 70% of the first use after ten cycles. The catalyzed capability of immobilized HRP was investigated and the immobilized HRP exhibited broader pH stability and excellent reusability. The results show that the Fe 3 O 4 /CRGO nanocomposites are appropriate for the immobilization of enzyme, and could have potential use in practical. |
| Databáze: | OpenAIRE |
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