Covalent immobilization of glucose oxidase on poly[1-(2-carboxyethyl)pyrrole] film for glucose sensing

Autor:	Shinnosuke Miyauchi, Masato Shimomura, Katsunori Kojima, Takeshi Yamauchi
Rok vydání:	1998
Předmět:	Polymers and Plastics biology Immobilized enzyme Organic Chemistry Condensation reaction humanities Amperometry Polyelectrolyte chemistry.chemical_compound chemistry Covalent bond Polymer chemistry Materials Chemistry biology.protein Glucose oxidase Biosensor Pyrrole
Zdroj:	Polymer. 39:2079-2082
ISSN:	0032-3861
DOI:	10.1016/s0032-3861(97)00531-4
Popis:	Poly[1-(2-carboxyethyl)pyrrole] film (PPy-COOH film) was electrochemically prepared and glucose oxidase (GOD) was covalently immobilized on the PPy-COOH film by the condensation reaction with carboxyl groups of the PPy-COOH. The apparent activity of the GOD immobilized on the PPy-COOH film was 152 mU cm −2 . The GOD-immobilized PPy-COOH film (PPy-GOD film) was applied to amperometric glucose sensing. Plots of response current versus glucose concentration gave a straight line with a slope of 1.7 μ A cm −2 per 1 mM glucose up to 80 mM. The glucose oxidation with the GOD on the PPy-GOD film was considered to proceed through a Michaelis—Menten mechanism.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::04524768b174c76523508067f4a2141f https://doi.org/10.1016/s0032-3861(97)00531-4 Zobrazit plný text záznamu Full Text from ScienceDirect