In VitroRelease of ACTH: Effects of Potassium, Calcium and Corticosterone1

Autor:	C. M. Branson, Jacob Kraicer, J. L. Gosbee, R. G. Conrad, John V. Milligan
Rok vydání:	1969
Předmět:	endocrine system medicine.medical_specialty Bicarbonate Potassium chemistry.chemical_element Calcium Adenohypophysial Cell In vitro chemistry.chemical_compound Endocrinology chemistry Corticosterone Internal medicine medicine Secretion Hormone
Zdroj:	Endocrinology. 85:1144-1153
ISSN:	1945-7170 0013-7227
DOI:	10.1210/endo-85-6-1144
Popis:	Adenohypophyses were incubated in Krebs-Ringer bicarbonate and the release of ACTH was measured under various conditions. A 5-fold increase in K+ augmented ACTH release. This augmented release was significantly greater from adenohypophyses of adrenalectomized rats than from those of intact animals and was prevented when Ca++ was removed from, or corticosterone was added to, the incubating medium. The increased release of ACTH in the high K+ medium and its prevention when Ca++ was removed were reversible and repeatable. These findings are consistent with the model of “excitation- secretion coupling” which has been proposed to describe the process of hormone release. These findings also suggest that corticosterone may operate directly on releasing mechanisms, possibly in the adenohypophysial cell membrane, to inhibit ACTH release. (Endocrinology 85: 1144, 1969)
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0446a83cc73b3153a6948f4428dfda41 https://doi.org/10.1210/endo-85-6-1144 Zobrazit plný text záznamu