Direct determination of single-to-double stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye–DNA complexes

Autor:	Juan C. Scaiano, Gonzalo Cosa, J. R. N. McLean, K.-S. Focsaneanu
Rok vydání:	2000
Předmět:	Chemistry Direct method Metals and Alloys Analytical chemistry General Chemistry Fluorescence Catalysis Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Materials Chemistry Ceramics and Composites Time-resolved spectroscopy Double stranded DNA
Zdroj:	Chemical Communications. :689-690
ISSN:	1364-548X 1359-7345
DOI:	10.1039/b000473l
Popis:	We report the fluorescence lifetimes of the DNA-stain dye PicoGreen and discuss the difference exhibited in the upon binding to single-stranded vs. double-stranded DNA; we here developed a direct method for determining single-to-double stranded DNA ratios in solution by measurement of the pre-exponential factors in the fluorescence decay traces of dye–DNA complexes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::043fae7e5cfb5ddedb28db1095fab3c1 https://doi.org/10.1039/b000473l Zobrazit plný text záznamu