nifGene expression in a Nif+, Fix−Bradyrhizobium japanicumvariant

Autor:	James A. Bradburne, James N. Mathis, Daniel W. Israel
Rok vydání:	1994
Předmět:	Rhizobiaceae biology Nif gene Nitrogenase biology.organism_classification Microbiology Molecular biology Bradyrhizobium Botany Genetics Nitrogen fixation Northern blot Molecular Biology Bacteria Bradyrhizobium japonicum
Zdroj:	FEMS Microbiology Letters. 123:91-98
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1994.tb07206.x
Popis:	Bradyhizobium japonicum USDA 110 has been shown to contain several genetically similar, naturally occurring colony morphology variants. One of these variants, L2-110, although devoid of symbiotic nitrogen fixation, retains significant levels of explanta nitrogen fixation ability relative to other symbiotically competent USDA 110 variants (MN-110 and I-110). Interestingly, Northern blot analyses revealed that L2-110 nodules, despite their lack of symbiotic nitrogen fixation, contained 65% the level of mRNA for dinitrogenase (nifDK) and 64% the level of dinitrogenase reductase (nifH) mRNA relative to MN-110 nodules. Western blot analyses of tissue from the same nodules detected 32% the level of dinitrogenase and 31% the level of dinitrogenase reductase in L2-110 relative to MN-110. L2-110 appears to be a new class of mutant based on the complete absence of symbiotic nitrogen fixation (Fix− and the presence of significant exanta nitrogen fixation (Nif+).
Databáze:	OpenAIRE
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