Prevalence of O25b-ST131Escherichia coliClone: Fecal Carriage of Extended-Spectrumβ-Lactamase and Carbapenemase—Producing Isolates in Healthy Adults in Tehran, Iran
| Autor: | Mohammad Mehdi Aslani, Masoumeh Rasoulinasab, Mina Ebrahimi-Rad, Fereshteh Shahcheraghi, Azade Hajihasani |
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| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
Pharmacology Molecular epidemiology Avibactam Immunology Prevalence Ceftazidime Biology medicine.disease_cause Microbiology law.invention chemistry.chemical_compound chemistry law medicine Multilocus sequence typing Typing Escherichia coli Polymerase chain reaction medicine.drug |
| Zdroj: | Microbial Drug Resistance. 28:210-216 |
| ISSN: | 1931-8448 1076-6294 |
| Popis: | Fecal carriage of multidrug-resistant Escherichia coli, particularly sequence type 131 (ST131), is becoming a global concern. This study aimed at determining the prevalence rate and molecular epidemiology of extended-spectrum β-lactamase-producing E. coli (ESBL-Ec), carbapenemase-producing E. coli (CPEc), ceftazidime/avibactam (CAZ/AVI)-resistant E. coli, and ST131 isolates in healthy fecal carriers in Tehran, Iran. Among 540 samples studied, 233 (43.1%) carried ESBL-Ec, with the majority (93.9%) harboring the blaCTX-M. The carriage rate of CPEc was 2.5% (n = 14/540), and blaNDM gene was the predominant carbapenemase gene. Most CPEc isolates (n = 11/14) was shown to be resistant to CAZ/AVI. Among ESBL-Ec/CPEc, 7.3% (n = 17/233) belonged to E. coli ST131 clone, which was identified by polymerase chain reaction and confirmed by multilocus sequence typing. The ST131 isolates genetically typed by pulsed-field gel electrophoresis were heterogeneous and four different plasmids were detected by plasmid typing, with the IncFIA/FIB being the major type. Our findings disclose that the presence of carbapenem-resistant ST131 isolates, which are also resistant to CAZ/AVI, contributes to the spread of resistant strains in the community. Therefore, screening and monitoring of such resistant clone in healthy people is necessary. |
| Databáze: | OpenAIRE |
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