| Popis: |
The human microbiome has been studied extensively across sites in the body that are readily accessible to sampling. Internal organs and tissues, however, have remained largely unexplored and, in the absence of infectious disease, are widely assumed to be free of microorganisms. Using single-cell transcriptomic data from the Tabula Sapiens spanning 15 human organ donors, 20 tissues, 400,000+ annotated cells, 100+ cell types, and ∼70 billion sequences, we created an atlas of the healthy human tissue microbiome with cell type resolution. In order to construct this atlas, we developed a computational pipeline which identifies high-confidence microbial sequences from three domains of life within the sea of human transcripts in individual cells, and show that this is a non-trivial search for one in a million sequences. Together with data from a separate validation cohort of 8 additional donors, we identify more than a thousand diverse bacterial, viral and fungal species in human tissues. In characterizing the human tissue microbiome, we demonstrate that individuals harbor unique species associated not just with each tissue but often with particular cell types, and that many of these species remain uncharacterized. Moreover, combining our data with the Human Microbiome Project, the tumor microbiome dataset by Nejmanet al., and the PATRIC database, we map the likely microbial flow routes from external-facing microbiomes to internal tissues and tumors, and show the existence of many unexpected routes taken by both commensals and pathogens. We find that ∼30% of bacterial species found in tumors are detectable across healthy tissues, suggesting that tumor microbiomes are in part sourced from healthy tissues, even those from distant sites. Increasing the resolution of sampling from tissues to cell types, we quantify the microbial load and diversity across different cell types to reveal a network of host cell type and microbe associations. For example, we identified traces of both latent and active Epstein Barr Virus infections in various cell types such as splenic plasma cells. Broad exploration of the healthy tissue microbiome may provide insights which ultimately are of clinical importance. |
