Construction of intergeneric conjugal transfer for molecular genetic studies of Streptomyces acidiscabies producing thaxtomin

Autor:	Bo-Youn Jang, Yong-Il Hwang, Sun-Uk Choi, Hye-Yun Park
Rok vydání:	2012
Předmět:	fungi Organic Chemistry Biology medicine.disease_cause biology.organism_classification Genome General Biochemistry Genetics and Molecular Biology Homology (biology) Microbiology Spore Open reading frame Tuber crops Streptomyces acidiscabies medicine Bioorganic chemistry Escherichia coli
Zdroj:	Journal of the Korean Society for Applied Biological Chemistry. 55:265-269
ISSN:	2234-344X 1738-2203
DOI:	10.1007/s13765-012-1037-5
Popis:	Effective transformation procedure to facilitate molecular genetic studies of Streptomyces acidiscabies producing thaxtomin, which causes scab diseases in the economically important root and tuber crops was established via transconjugation from Escherichia coli ET12567 using an OC31-derived integration vector, pSET152, harboring the oriT and attP fragments. Greatest number of exconjugants was achieved on MS medium containing 50 mM MgCl2 without heat treatment of spores and E. coli cells. Additionally, the integration site, attB, of the genome of S. acidiscabies exhibited the highest degree of homology with S. avermitilis and its chromosomal location was found to exist as a single attB site within an open reading frame coding for a pirin homolog similar to those identified in other actinomycetes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0401ab7f19ae6d25cd43047f7420543c https://doi.org/10.1007/s13765-012-1037-5 Zobrazit plný text záznamu