The presence of functional haem in a higher plant nitrate reductase
| Autor: | R.J. Fido, B.A. Notton, Eric J. Hewitt |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Soil Science Dehydrogenase Electron acceptor Nitrate reductase biology.organism_classification chemistry.chemical_compound Affinity chromatography Biochemistry Nitrate Spinach Agarose Dichlorophenolindophenol Agronomy and Crop Science |
| Zdroj: | Plant Science Letters. 8:165-170 |
| ISSN: | 0304-4211 |
| DOI: | 10.1016/0304-4211(77)90027-x |
| Popis: | Nitrate reductase from spinach (Spinacea oleracea L. v. Noorman) has been purified 2600-fold by a multistep procedure involving streptomycin sulphate treatment, (NH4)2SO4 precipitation, hydroxylapatite adsorption, molecular sieve chromatography and blue-dextran agarose affinity chromatography. Spectral studies of the purified enzyme indicate the presence of a b-type cytochrome associated with the enzyme and involved in both its overall function of reducing nitrate with NADH and its dehydrogenase function using dichlorophenolindophenol (DCPIP) as electron acceptor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|