Enhancing hybrid Liquidambar somatic seedling production using a temporary immersion bioreactor
| Autor: | Siran Lu, Scott A. Merkle |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Ecology biology Liquidambar formosana Somatic embryogenesis Physiology Liquidambar styraciflua Liquidambar Forestry Plant Science biology.organism_classification 01 natural sciences Acclimatization Horticulture Seedling Germination 010608 biotechnology 010606 plant biology & botany Hybrid |
| Zdroj: | Trees. 35:503-512 |
| ISSN: | 1432-2285 0931-1890 |
| DOI: | 10.1007/s00468-020-02052-0 |
| Popis: | Temporary immersion bioreactors can improve hybrid sweetgum somatic embryo conversion and somatic seedling growth rates compared to semisolid medium, significantly improving somatic seedling production efficiency for hybrid sweetgum, an emerging woody biomass crop. Fast-growing hybrid southern hardwood trees should make excellent material for woody biomass production in the Southeastern US, if elite clones can be identified and efficiently propagated. We have enhanced the potential of sweetgum (Liquidambar styraciflua) as a biomass species by generating hybrids between the tree and its Chinese relative, Liquidambar formosana, and propagating the most promising clones via somatic embryogenesis. Some of the hybrid clones have already demonstrated superior biomass productivity compared to elite L. styraciflua trees. However, production of somatic seedlings from these clones remains labor-intensive. Bioreactors, specifically temporary immersion designs, such as the RITA®, have been applied to improve the efficiency of in vitro propagation of a number of woody species. We tested RITA® bioreactors for their potential to improve the production efficiency of high-quality hybrid sweetgum somatic seedlings. In one tested genotype, a RITA® with 50 somatic embryos had about 66% higher conversion frequency (p |
| Databáze: | OpenAIRE |
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