Isolation of Egg and Sperm Cells from Bauhinia blakeana
| Autor: | Lu Ming Fang, Shun De Su, Hui Qiao Tian, Ren Hua Zheng, Hui Xiao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Egg cell In vitro fertilisation Somatic cell medicine.medical_treatment Bauhinia × blakeana Plant Science Biology medicine.disease_cause biology.organism_classification 01 natural sciences Sperm Cell biology 03 medical and health sciences 030104 developmental biology Human fertilization medicine.anatomical_structure Pollen Genetics medicine Ovule 010606 plant biology & botany |
| Zdroj: | Tropical Plant Biology. 13:353-358 |
| ISSN: | 1935-9764 1935-9756 |
| Popis: | Isolated sperm cells and egg cells provide useful materials for the study of fertilization in higher plants, under controlled conditions, in the absence of somatic tissue. Viable egg cells from Bauhinia blakeana were isolated using enzymatic digestion and mechanical dissection. Ovules were digested with enzymes for 40–50 min and then transferred to a solution without enzymes for dissection. The three cells of the egg apparatus (i.e., the egg cell and two synergids) were released from a cut ovule by pushing the micropylar region of the ovule with a dissecting needle. Typically, 7–10 egg cells were isolated within 1 h. B. blakeana pollen is bicellular and contains only a vegetative and generative cell at anthesis. Sperm cells were isolated using an in vivo–in vitro method. The isolation of B. blakeana egg and sperm cells potentially provides a basis for in vitro fertilization of this woody dicotyledonous plant and materials for molecular biological investigations of fertilization mechanisms. |
| Databáze: | OpenAIRE |
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