Growth, substrate consumption, and product formation kinetics of Phanerochaete chrysosporium and Schizophyllum commune mixed culture under solid-state fermentation of fruit peels
| Autor: | Md. Zahangir Alam, Kola Saheed Olorunnisola, Parveen Jamal |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
biology
Chemistry Schizophyllum commune Substrate (chemistry) Cellulase Environmental Science (miscellaneous) Bacterial growth biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Solid-state fermentation biology.protein Phanerochaete Fermentation Food science Biotechnology Chrysosporium |
| Zdroj: | 3 Biotech. 8 |
| ISSN: | 2190-5738 2190-572X |
| DOI: | 10.1007/s13205-018-1452-3 |
| Popis: | Kinetic analysis of solid-state fermentation (SSF) of fruit peels with Phanerochaete chrysosporium and Schizophyllum commune mixed culture was studied in flask and 7 kg capacity reactor. Modified Monod kinetic model suggested by Haldane sufficiently described microbial growth with co-efficient of determination (R2) reaching 0.908 at increased substrate concentration than the classical Monod model (R2 = 0.932). Leudeking–Piret model adequately described product synthesis in non-growth-dependent manner (R2 = 0.989), while substrate consumption by P. chrysosporium and S. commune fungal mixed culture was growth-dependent (R2 = 0.938). Hanes–Woolf model sufficiently represented α-amylase and cellulase enzymes synthesis (R2 = 0.911 and 0.988); α-amylase had enzyme maximum velocity (Vmax) of 25.19 IU/gds/day and rate constant (Km) of 11.55 IU/gds/day, while cellulase enzyme had Vmax of 3.05 IU/gds/day and Km of 57.47 IU/gds/day. Product yield in the reactor increased to 32.65 mg/g/day compared with 28.15 mg/g/day in shake flask. 2.5 cm media thickness was adequate for product formation within a 6 day SSF in the tray reactor. |
| Databáze: | OpenAIRE |
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