Use of alacZfusion to study transcriptional regulation of theRhodobacter capsulatus hemAgene

Autor:	John J. Eckert, Maureen S. Wright, Susan W. Biel, A J Biel
Rok vydání:	1991
Předmět:	Regulation of gene expression Rhodobacter lac operon Biology biology.organism_classification Microbiology Molecular biology Fusion protein Oxygen tension Plasmid Genetics Transcriptional regulation Molecular Biology Gene
Zdroj:	FEMS Microbiology Letters. 78:339-342
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1991.tb04466.x
Popis:	An EcoRI fragment containing the Rhodobacter capsulatus hemA promoter has been cloned into a lacZ translational fusion vector. The resulting plasmid produced a hemA-lacZ fusion protein with a molecular mass of 147,000. Expression of the hemA-lacZ fusion, as measured by production of beta-galactosidase, was regulated 2- to 3-fold by oxygen tension. The unexpectedly small change in beta-galactosidase levels suggests that transcriptional regulation of the hemA gene is not the major factor in oxygen-mediated control of porphyrin synthesis.
Databáze:	OpenAIRE
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