| Autor: |
Gopesh Srivastava, Iain A. Borthwick, Brian K. May, D. J. Maguire, William H. Elliott, Cornelis J. Elferink |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Journal of Biological Chemistry. 262:3988-3992 |
| ISSN: |
0021-9258 |
| DOI: |
10.1016/s0021-9258(18)61300-6 |
| Popis: |
Reports to date have led to the conclusion that there are isozymes for 5-aminolevulinate synthase in the liver and erythroid tissue of chicken. Indeed, the existence of a multigene family for chicken 5-aminolevulinate synthase has been proposed. We find no evidence to support these proposals. In this work we show that 5-aminolevulinate synthase mRNA from chicken liver and reticulocytes is identical as determined by RNase mapping and primer extension studies and that the 5-aminolevulinate synthase protein from these tissues is the same size as judged by immunoblot analysis. We also show that a single mRNA species for 5-aminolevulinate synthase is present in chicken liver, reticulocytes, brain, and heart and an avian erythroblastosis virus-transformed chicken erythroblast cell line. Southern analysis shows the presence of only one gene copy for 5-aminolevulinate synthase in the chicken haploid genome. Overall, these results lead to the conclusion that in chickens 5-aminolevulinate synthase is encoded by a unique gene and is expressed as a single mRNA species in all tissues. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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