A SIMPLE, RAPID AND SIMULTANEOUS PREPARATION OF GLANDLESS COTTENSEED 7S AND 11S PROTEIN FRACTIONS AND CHARACTERIZATION OF SOME PHYSICOCHEMICAL PROPERTIES

Autor:	K. C. Cho, P. S. W. Park, E. T. Adams, K. C. Rhee
Rok vydání:	1992
Předmět:	Gel permeation chromatography Electrophoresis Column chromatography Chromatography Chemistry Osmometer Size-exclusion chromatography Ion chromatography Ultracentrifuge Safety Risk Reliability and Quality Polyacrylamide gel electrophoresis Food Science
Zdroj:	Journal of Food Quality. 15:357-367
ISSN:	1745-4557 0146-9428
DOI:	10.1111/j.1745-4557.1992.tb00962.x
Popis:	A simple and rapid method for simultaneous preparation of individual protein fractions from glandless cottonseed storage proteins was developed. With gel filtration chromatography, using Sephacryl S-300 HR and 10% NaCl solution as an eluent, 3 fractions, FI, FII, FIII were obtained. FII and FIII produced single peaks by gel filtration rechromatography and anion exchange chromatography. FII and FIII showed both a single major and a barely noticeable minor band, respectively, on polyacrylamide gel electrophoresis. Analytical ultracentrifuge patterns showed impurites less than 2% in FII and a single symmetrical peak for FIII. Sedimentation coefficients were computed to be 10.7S and 6.8S for FII and FIII, respectively; molecular weights by osmometry were 2.39 × 105 and 1.40 × 105 g/mole for FII and FIII, respectively. Both fractions were high in acidic and low in sulfur-containing amino acids, and contained phytate and gossypol at less than 10 and 2 ppm, respectively.
Databáze:	OpenAIRE
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