Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia
| Autor: | Olga Garaschuk, Bianca Brawek |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microglia Chemistry Electroporation Cell Dynamics (mechanics) 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure nervous system Two-photon excitation microscopy Calcium dynamics In vivo medicine Biophysics 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Microglia ISBN: 9781493996575 |
| Popis: | Similar to many nonexcitable cells, microglia utilize intracellular Ca2+ signaling for the communication with each other as well as neurons and astrocytes and for triggering a magnitude of their executive functions. However, in vivo measurements of the intracellular Ca2+ dynamics in microglia have been challenging due to technical reasons. Here, we describe an approach utilizing a single-cell electroporation technique to facilitate the study of microglial Ca2+ signaling in the living brain. |
| Databáze: | OpenAIRE |
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