Automated particle-counting immunoassay for alpha-fetoprotein

Autor:	C G Magnusson, J. G. Ratcliffe, Cesar Cambiaso, D Collet-Cassart, Pl. Masson
Rok vydání:	1981
Předmět:	Antiserum Chromatography Correlation coefficient biology medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Immunoglobulin G Standard curve Agglutination (biology) Immunoassay biology.protein medicine Particle
Zdroj:	Clinical Chemistry. 27:64-67
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/27.1.64
Popis:	PACIA, a homogeneous non-radioimmunoassay, has been adapted to the determination of serum alpha 1-fetoprotein. This technique is based on the agglutination of latex particles coated with antibodies to the antigen to be determined. The agglutination is measured by using an optical cell counter designed to count blood cells, to determine the reduction in the number of non-agglutinated particles. Interferences by serum constituents are avoided by coating the particles with the F(ab')2-fragments of th immunoglobulin G fraction of the antiserum. The system is automated, with a sampling rate of 50/h and an incubation time of 26 min. Concentrations used in preparing the standard curve ranged from 1 to 50 microgram/L; analytical recoveries were 93.5 to 98.4%; the correlation coefficient of PACIA with radioimmunoassay, calculated from results on 127 samples, was 0.98; maximum within- and between-assay CVs were 7.4% and 9.6%, respectively.
Databáze:	OpenAIRE
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