Analysis of the genetic structure of Tuvinian short-fat-tailed sheep populations with the use of the ISSR-PCR method

Autor: Yu. A. Stolpovsky, L. V. Shimiit, A. N. Evsyukov, M. N. Ruzina, N. V. Kol, G. E. Sulimova
Rok vydání: 2010
Předmět:
Zdroj: Russian Journal of Genetics. 46:1462-1470
ISSN: 1608-3369
1022-7954
DOI: 10.1134/s1022795410120094
Popis: The genetic structure of populations of the Tuvinian short-fat-tailed sheep was studied with the use of the ISSR-PCR (Inter Simple Sequence Repeats) method in 18 farms of Tyva. Data on the spectrum of ISSR fragments of DNA were obtained using the (AG)9C primer. Analysis of intermicrosatellite polymorphism permitted us to determine genomic characteristics of the populations, their genealogical relations, and the parameters of genetic diversity within the populations and the breed as a whole. Three genetic notions were considered on the basis of the results of this analysis: gene pool profile, gene pool standard, and breed-specific pattern. The data obtained can be used to carry out population genetic monitoring, to develop a breeding strategy, and to conserve in situ the Tuvinian sheep breed and breeds of other domesticated species.
Databáze: OpenAIRE