A practical method for partial purification of the organism of psittacosis grown in chicken embryo yolk sac

Autor:	Warren G. Dorsey, William N. Shirey, William C. Patrick
Rok vydání:	1965
Předmět:	Antigenicity Chromatography Sucrose chemistry.chemical_element Bioengineering Embryo Metabolism Biology Calcium Applied Microbiology and Biotechnology chemistry.chemical_compound medicine.anatomical_structure chemistry Biochemistry medicine Centrifugation Yolk sac Digestion Biotechnology
Zdroj:	Biotechnology and Bioengineering. 7:447-454
ISSN:	1097-0290 0006-3592
DOI:	10.1002/bit.260070402
Popis:	A procedure described for the partial purification of suspensions of the organisms of psittacosis utilizes tryptic digestion, calcium chloridedextran sulfate precipitation, and centrifugation through a sucrose barrier. More than 40% of the organisms in the original suspension are recovered in 80% of the original volume, with approximately 95% of the lipids and 85% of the proteins removed. This method is suggested as a preliminary purification procedure in laboratories desiring relatively large amounts of purified agent for studies on metabolism, physical properties, or antigenicity of the organism. Significant aspects of the investigations leading to the selection of the procedures are included.
Databáze:	OpenAIRE
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