Ecophysiological, anatomical and biochemical aspects of in vitro culture of zygotic Syagrus coronata embryos and of young plants under drought stress

Autor: Laureen Michelle Houllou, Maria Jaislanny Lacerda e Medeiros, Marciel T. Oliveira, Mauro Santos, Lilia Willadino, Déborah Silva Oliveira
Rok vydání: 2015
Předmět:
Zdroj: Trees. 29:1219-1233
ISSN: 1432-2285
0931-1890
DOI: 10.1007/s00468-015-1202-7
Popis: The in vitro culture of zygotic embryos is an efficient alternative to overcome dormancy of Syagrus coronata and young plants showed high resilience during drought and rehydration. A method for obtaining plants from the cultivation of zygotic embryos of the species Syagrus coronata (Mart.) Becc. was evaluated, and rates of contamination, oxidation, germination and normal plants and the use of pre-acclimatization treatments involving modifications of culture vessel lids were assessed. Anatomical descriptions of the embryos and plants during the initial stages of development were also performed. Additionally, the mechanisms of drought tolerance, water relations, gas exchange, chlorophyll fluorescence and biochemical parameters from the plants were evaluated during drought and recovery. In the first week of in vitro culture, we observed the intumescense of the embryo, suggesting that dormancy is not related to maturity level. Anatomically, the embryonic axis was located at the embryo’s proximal region, while the distal region contained the haustorium. The use of Y3 medium with activated charcoal or lauric acid resulted in the highest number of plants, and the pre-acclimatization treatment (one 95 mm2 opening in the lid) allowed for a 100 % survival of the plants under ex vitro conditions. The plants adjusted their metabolic rates during water deficit by maintaining their leaf water concentrations, robust stomatal control and high rates of non-photochemical energy dissipation. Moreover, during recovery, the plants rapidly reached their photosynthetic capacities. This behavior indicates that the use of in vitro culture does not compromise the plants’ performance, which was indicated by the high resiliencies they displayed during the experimental period.
Databáze: OpenAIRE
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