NIR and 1H qNMR methods coupled to chemometrics discriminate the chemotypes of the gastroprotective herb Egletes viscosa

Autor: Kirley Marques Canuto, Kaline R. Carvalho, Edy Sousa de Brito, Guilherme Julião Zocolo, Lorena Mara A. Silva, Ebenézer de Oliveira Silva, Ana Sheila de Queiroz Souza, Elenilson G. Alves Filho, Edilberto R. Silveira, Rita de Cássia Alves Pereira
Rok vydání: 2020
Předmět:
Zdroj: Food Research International. 138:109759
ISSN: 0963-9969
DOI: 10.1016/j.foodres.2020.109759
Popis: Egletes viscosa is a Brazilian medicinal herb consumed as flower bud tea due to its gastroprotective properties. This plant possesses two essential oil-based chemical varieties: trans-pinocarveyl acetate-rich chemotype A and cis-isopinocarveyl acetate- rich chemotype B. Therefore, we developed two simple, fast and reliable methods for discrimination of E. viscosa chemotypes using NIR and 1H qNMR spectroscopies combined with the chemometrics tools (iPLS and PLS-DA). Both methods showed high sensitivity, precision and specificity in the cross-validation tests. The NIR method has the advantages of being non-destructive and analyzable by portable devices, enabling its application for field and industrial evaluations. Meanwhile, the 1H qNMR method allows the quantification of the bioactive components ternatin, tanabalin, and centipedic acid. These aforementioned compounds were found higher in the chemotype A. Accordingly, our methods showed to be complimentary approaches for authenticity and/or quality control of E. viscosa-derived raw materials and herbal products.
Databáze: OpenAIRE
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