| Autor: |
U. Andrae, U. Forster, Helmut Greim, L.R. Schwarz |
| Rok vydání: |
1986 |
| Předmět: |
|
| Zdroj: |
Archives of Toxicology ISBN: 9783540165897 |
| DOI: |
10.1007/978-3-642-71248-7_32 |
| Popis: |
There is increasing application of in vitro-test systems for toxicological evaluation of chemicals, which became possible by increasing understanding of the biological endpoints present in such systems and their capability for metabolic activation and inactivation. This communication focusses on the capacities of metabolic activation and inactivation in mutagenicity test systems, using bacteria, mammalian cells in culture and isolated hepatocytes. Bacterial test procedures with S-9-fraction are specific metabolic activation systems with low inactivation capacity. Mammalian cells are either deficient in metabolic activities or contain only limited activation capacity although special cell lines derived from hepatoma cells express certain metabolic activation as well as inactivation reactions. Isolated hepatocytes contain enzymatic activities similar to those in the intact liver, which however decrease at different rates. It is the goal of present research to construct cell lines with defined and sufficient activities of these enzymes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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