Nachweis von Weizengliadinen in erhitzten Lebensmitteln mittels Phasenumkehr-Hochdruckfl�ssig-Chromatographie
| Autor: | Peter Meier, Erich Baumgartner, Helena Windemann |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
food.ingredient
biology Chemistry digestive oral and skin physiology fungi Wheat flour food and beverages General Chemistry Biochemistry High-performance liquid chromatography Industrial and Manufacturing Engineering Quantitative determination food Skimmed milk biology.protein Heat treated Food components Food science Gliadin Food Science Biotechnology |
| Zdroj: | Zeitschrift f�r Lebensmittel-Untersuchung und -Forschung. 183:26-32 |
| ISSN: | 1438-2385 0044-3026 |
| DOI: | 10.1007/bf01027590 |
| Popis: | To recognize and determine the wheat gliadins in unheated gluten-free food for coeliac patients the immunological methods such as ELISA can be used. In heated food (above 80 degrees-90 degrees C) these methods fail wholly or in part to achieve the quantitative determination of wheat gliadin. The changes in protein patterns after heat treatment are also revealed by the RP-HPLC of wheat gliadins and some peaks appear, which are characteristic for heat treated wheat flour. Using these peaks, about 2% admixture of wheat flour (T. aestivum, T. durum) as well as of rye flour can be detected. In foods which contain more than 50% skim milk the addition of only at least 5% of these flours can be detected. The ethanolic extracts of foods were concentrated by freeze-drying prior to analysis by HPLC. The ethanol-soluble non-dialysable food components affect the quantitative determination of wheat or rye proteins by means of peak areas. Selective enrichment is a possibility. The RP-HPLC-analysis of ethanol-soluble proteins makes it possible to detect heated flours of wheat and rye (cooked, roller-dried, extrusion-cooked) in glutenfree food. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink