In vitroandin vivostudies of a gelatin/carboxymethyl chitosan/LAPONITE® composite scaffold for bone tissue engineering
| Autor: | Liu Zhonglong, Zhou Xiao-jun, Li Tao, Yang Zezheng, Liu Zhiyuan, Wang Jinwu, Xiao Ming |
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| Rok vydání: | 2017 |
| Předmět: |
Scaffold
food.ingredient Chemistry General Chemical Engineering Mesenchymal stem cell 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Gelatin In vitro 0104 chemical sciences food In vivo Biophysics medicine Swelling medicine.symptom Biocomposite 0210 nano-technology Bone regeneration |
| Zdroj: | RSC Advances. 7:54100-54110 |
| ISSN: | 2046-2069 |
| Popis: | In the present study, we fabricated a biocomposite scaffold composed of carboxymethyl chitosan (CMC), gelatin and LAPONITE® (Lap) nanoparticles via freeze-drying and investigated its potential use in bone tissue engineering. The prepared gelatin/carboxymethyl chitosan (GC) scaffolds and laponite-incorporated scaffolds were characterized by scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) analyses. The swelling and biodegradation were also investigated. In vitro assays such as cell attachment and proliferation, osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMCSs) grown on those scaffolds and in vivo cranial bone defect assays were further carried out. We found that our prepared scaffolds had a porous architecture, and the increased Lap content resulted in improved mechanical strength, whereas the swelling ratio and degradation rate decreased. In vitro cell proliferation and live cell staining experiments demonstrated that the addition of Lap (5 and 10 wt% relative to gelatin, GC-Lap5% and GC-Lap10% respectively) would facilitate cell proliferation, but caused an inhibition effect at 15% of Lap content (GC-Lap15%). Furthermore, GC-Lap10% induced a higher degree of osteogenic differentiation of rBMSCs compared with the GC scaffold and GC-Lap5% scaffold. More importantly, in vivo cranial defect experiments revealed that the addition of Lap into the GC scaffold promoted bone regeneration. These findings indicate that a composite scaffold with Lap incorporation is a promising material for bone tissue engineering. |
| Databáze: | OpenAIRE |
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