Autor: | Peter A. Steck, Samar A. Jasser, Jeffrey C. Sung, W. K. Alfred Yung, Yuexi Shi, Ping Tang |
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Rok vydání: | 1999 |
Předmět: |
Cancer Research
Cell growth Biology medicine.disease Molecular biology Reverse transcriptase chemistry.chemical_compound Neurology Oncology chemistry Glioma Gene expression Sense (molecular biology) Cancer research medicine Neurology (clinical) Growth inhibition Autocrine signalling Transforming growth factor |
Zdroj: | Journal of Neuro-Oncology. 43:127-135 |
ISSN: | 0167-594X |
Popis: | The effects of transforming growth factor-α (TGF-α) on cell growth were studied in human glioma U251 cells transfected with antisense TGF-α vectors (pcDNAI.neo). Several antisense clones showed a marked decrease in growth rate in serum-free medium but not in medium containing 10% FBS, compared with those of parental cells and clones from sense or vector transfectants. Antisense clones also produced fewer and smaller colonies in anchorage-independent growth assays. Moreover, there was a reduction in TGF-α expression in these antisense clones at both the protein and mRNA levels, as determined by enzyme linked immuno-sorbent assay and reverse transcriptase polymerase chain reaction analysis. A U251 clone transfected by TGF-α antisense in a different vector (pMT/Ep) also showed a marked suppression in cell growth and TGF-α mRNA level. Finally, transfected clones with either vector system, showed decreased tumorigenicity in nude mice. In summary, a strong correlation between the inhibition of glioma cell growth and TGF-α expression was obtained from two different plasmid vectors, indicating that the expression of TGF-α could be specifically and effectively down-regulated by TGF-α antisense vector, which in turn led to growth inhibition. These studies suggests that TGF-α plays an essential role in controlling human glioma cell proliferation and may serve as a potential target for treatment of malignant glioma. |
Databáze: | OpenAIRE |
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