Terpenoid Metabolism in Plastids

Autor: Judy Brangeon, René Monéger, Bilal Camara, Odette Dogbo, Françoise Bardat
Rok vydání: 1983
Předmět:
Zdroj: Plant Physiology. 73:94-99
ISSN: 1532-2548
0032-0889
Popis: A technique for the isolation and the purification of Capsicum annum L. var. Yolo Wonder chromoplasts is described. The degree of purity of the isolated chromoplasts is greatly improved by the absence of MgCl2 in the extraction medium and in the gradient purification system, as shown by electron micrographs and the near absence of antimycin-insensitive NADH:cytochrome c reductase activity and succinate:cytochrome c reductase activity. Furthermore, phosphatidylserine was absent and the phosphatidylethanolamine content was reduced by a factor of 5 in these preparations, which were active in the synthesis of galactolipids, prenylquinones, and carotenoids.
Databáze: OpenAIRE