Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts
| Autor: | András Szilágyi, György Marosi, Diána Weiser, Zsombor Kristóf Nagy, Béla Koczka, László Poppe, Gergely Bánóczi, Ákos Gellért, Bálint Kiss, Péter L. Sóti, Viktória Bódai |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Vinyl alcohol
biology 010405 organic chemistry Organic Chemistry technology industry and agriculture Pseudomonas fluorescens macromolecular substances 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Biochemistry 0104 chemical sciences Kinetic resolution Acylation chemistry.chemical_compound Differential scanning calorimetry chemistry Biocatalysis Nanofiber Drug Discovery biology.protein Organic chemistry Lipase 0210 nano-technology |
| Zdroj: | Tetrahedron. 72:7335-7342 |
| ISSN: | 0040-4020 |
| Popis: | Immobilization of lipases from Pseudomonas fluorescens (Lipase AK), Burkholderia (Pseudomonas) cepacia (Lipase PS) and lipase B from Pseudozyma (Candida) antarctica (CaLB) was investigated by entrapment in electrospun poly(vinyl alcohol) (PVA) nanofibers. The activity and selectivity of the lipases entrapped in PVA nanofibers were characterized in kinetic resolution of racemic secondary alcohols using acylation in organic media. Potential bioimprinting effect of eight substrate-mimicking additives [polyethylene glycols (PEGs), non-ionic detergents (NIDs) and various organosilanes] was tested with the fiber-entrapped lipases. The nanofibrous lipase biocatalyst entrapped in the presence of the additives were also characterized by rheology, differential scanning calorimetry and scanning electron microscopy. In addition to the known lipase-bioimprinting agents (PEGs, NIDs), phenyl- and octyltriethoxysilane also enhanced substantially the biocatalytic properties of lipases in their electrospun PVA fiber-entrapped forms. The reasons of bioimprinting effect of several additives were rationalized by docking studies in the open and closed form of CaLB. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect